Human mesenchymal stem cells
(hMSC) are currently in use in over 400 clinical trials
and are critical components of tomorrow’s cell-based products and devices (1, 2, 3). Secretion of biomolecules
by hMSC influences many biological processes and is thought to be central to the mechanism of action. Since widespread clinical use of hMSC
and cell-based therapies with positive economic outcomes
will be facilitated by frozen storage, cryopreserved hMSC must maintain high levels of biological function upon thaw. Additionally, while hMSC have an excellent clinical track record in terms of safety, efficacy data has been difficult to come by, suggesting that more standardized cell formats are needed. This too could be addressed by effective means of cryopreservation, allowing off-the-shelf hMSC products
to be widely used in Regenerative Medicine
, Tissue Engineering and for 3D BioPrinting of cells and tissues.