The RoosterKit™-hBM-1M-XF contains 500 mL of RoosterNourish™-MSC-XF and 1 vial of 1M cryopreserved hBM-MSCs (RoosterVial™-hBM-1M-XF, Part No. MSC-031). RoosterNourish™-MSC-XF is our translation-friendly, highly-productive, xeno-free (XF) bioprocess medium for the rapid expansion of human mesenchymal stem/stromal cells (hMSC) from multiple tissue sources. RoosterVial™-hBM-1M-XF (Part No. MSC-031) contains 1M cryopreserved hBM-MSC identical to our 10M cell product, RoosterVial™-hBM-10M-XF (Cat. No. MSC-030). Following the provided RoosterBio® protocols this affordable kit can generate >100M cells within 10 days. Lots are released based on hMSC identity (cell surface marker expression), expansion, viability, sterility, and differentiation and provided QC Brief containing industry-leading functional characterization (immunomodulatory potential (IDO assay), angiogenic cytokine secretion). It is ideal for small scale testing and optimization of experimental conditions for future scale-up, and manufactured with cGMP compatible processes providing a direct path to translation.
This kit has been used worldwide to test and optimize a process capable of generating high volumes of hMSC critical raw materials and has supported top regenerative medicine research, product developers, and translational institutions.
Supplied, easy-to-follow expansion protocols for rapid testing and optimization
> 10 fold expansion in 4 to 6 days
Built in batch culture manufacturing process capable of generating >100 M cells in 8 to 10 days
Multiple donors available for screening
Manufactured with same cGMP compatible processes as 10M vial (MSC-030)
1st in class CoA and QC Brief complete with lot specific functional hMSC characterization (identity, trilineage differentiation, immunomodulatory potential, and angiogenic cytokine secretion)
RoosterVial™-hBM-1M-XF (xeno-free, MSC-031) cultured in RoosterNourish™-MSC-XF (KT-016) have a fibroblastic cell morphology (left) and express hMSC cell surface markers (right).
Immunomodulatory activity (left, IDO activity increase in response to IFN-y) and angiogenic cytokine secretion of a representative RoosterVial™-hBM-1M-XF (MSC-031) lot (right).
Trilineage differentiation of RoosterVial™-hBM-1M-XF (MSC-031) of a representative RoosterVial™-hBM-1M-XF (MSC-031) lot. Adipogenesis, left; Osteogenesis, middle; Chondrogenesis, right.
