The Xeno-Free Bioreactor Scale-Up Bundle is designed for process development into the PBS Biotech 3L bioreactor, while maintaining consistent population doubling level of the final hMSC population. The bundle consists of a vial of 10M XF hMSCs, adequate XF bioprocess media to generate the seed stock and perform a 3L fed-batch expansion, and a 3L bioreactor vessel.
A PBS Biotech bioreactor controller is required to run this scale-up bundle. This must be ordered separately through PBS at email@example.com.
Per our protocols and process recommendations for XF bioreactor cell culture, RoosterBio suggests using Corning® Low Concentration Synthemax® II Microcarriers (3781). Our system is also compatible with Corning CellBIND® Microcarriers (3779). For this application, four (4) vials are required.