Recombinant Bovine PDGF-BB Protein
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Overview
Bovine PDGF-BB (platelet-derived growth factor BB) protein is a homodimer of PDGF-B peptide chains. PDGF-BB can be used for the development of optimized serum-free culture media for species-specific bovine in cellular agriculture protocols. PDGF-BB can be added to bovine culture medium to promote rapid and efficient cell division, maintain high cell proliferation rates in bioreactors, promote the maturation of bovine myoblasts into myotubes and stimulate the production of extracellular matrix components.
Qkine bovine PDGF-BB is a high-purity 24.5 kDa disulfide-linked bioactive dimeric protein that is animal origin-free (AOF) and carrier protein-free. Qkine has optimized the bovine PDGF-BB manufacturing process to produce a highly bioactive species-specific protein with excellent lot-to-lot consistency for enhanced reproducibility.
Download the Product Data Sheet for species reactivity, reconstitution instructions, bioactivity, purity, and additional product information.
Contact us for Quality and Safety documentation or for information on cell therapy grade growth factors and cytokines.
Product Features
- High purity bovine protein (Uniprot number: B1H0W5)
- 24.5 kDa (monomer)
- >98%, by SDS-PAGE quantitative densitometry
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Qkine’s Cambridge, UK laboratories
- Lyophilized from acetonitrile, TFA
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured Applications:
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- Culture of bovine (cow) muscle stem cells
- Cellular agriculture and cultivated meat cell culture media optimization
- Serum-free media development
- Proliferation of fibroblasts and smooth muscle cells
RoosterBio is a distributor of Qkine growth factors and cytokines within the Unites States, for regional specific orders please contact us for more information.
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How do I store my proteins?
- Lyophilized store at -20°C or -80°C for up to 2 years
- Reconstituted store at -20 °C or -80 °C
- We recommend that single use aliquots are prepared to avoid freeze-thaw cycles
How do I reconstitute lyophilized protein?
- Check the resuspension solution recommendation on the product datasheet.*
- Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
- Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
- Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating. Do not vortex and avoid foaming.
- Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles, which can damage the proteins and reduce bioactivity. Store aliquots at -80°C (or -20°C).**
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors, so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml), adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).