Recombinant Bovine/porcine FGF-2 (154 Aa) Protein
Recommended Products
There are no recommended products for Recombinant Bovine/porcine FGF-2 (154 Aa) Protein
Overview
Recombinant bovine/porcine FGF-2 protein 154 aa (bFGF/basic FGF), this is the long form of FGF-2 used for the development of optimized serum-free culture media for species-specific bovine (cow) and porcine (pig) cultivated meat and veterinary research applications. Used in comparative media optimization studies with bovine/porcine FGF-2 145 aa (Qk040).
FGF-2 is used extensively in the maintenance and proliferation of induced pluripotent (iPSC) and embryonic stem cells (ESC) and for enhancement of proliferation in primary cell culture.
High purity 16 kDa FGF-2 / bFGF 154 aa protein, animal origin-free (AOF) and carrier-protein free (CF).
Download the Product Data Sheet for species reactivity, reconstitution instructions, bioactivity, purity, and additional product information.
Contact us for Quality and Safety documentation or for information on cell therapy grade growth factors and cytokines.
Product Features
- High purity recombinant bovine/porcine FGF-2 protein (154 aa) / bFGF (Uniprot: P03969)
- 17.1 kDa
- >98%, by SDS-PAGE quantitative densitometry
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Qkine’s Cambridge, UK laboratories
- Lyophilized from Tris, NaCl, CyS, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured Applications:
-
- Bovine and porcine primary cell culture
- Bovine and porcine stem cell expansion and maintenance
- Cellular agriculture process development
- Serum-free media development
RoosterBio is a distributor of Qkine growth factors and cytokines within the Unites States, for regional specific orders please contact us for more information.
Login to search Certificates of Analysis and Quality Control Briefs.
How do I store my proteins?
- Lyophilized store at -20°C or -80°C for up to 2 years
- Reconstituted store at -20 °C or -80 °C
- We recommend that single use aliquots are prepared to avoid freeze-thaw cycles
How do I reconstitute lyophilized protein?
- Check the resuspension solution recommendation on the product datasheet.*
- Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
- Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
- Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating. Do not vortex and avoid foaming.
- Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles, which can damage the proteins and reduce bioactivity. Store aliquots at -80°C (or -20°C).**
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors, so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml), adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).