Recombinant Bovine/Porcine FGF2-G3 (145 aa) Protein
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Overview
Recombinant bovine/porcine FGF2-G3 protein is a thermostable engineered form of FGF-2 (bFGF). Qk080 is the thermostable form of the wild-type FGF-2 145 aa (Qk040). FGF2-G3 has an increased functional half-life from <10 h (wild-type) to >7 days (FGF2-G3). Bovine FGF2-G3 is used for the development of optimized serum-free culture media for species-specific bovine (cow) and porcine (pork) cultivated meat and veterinary research applications, including the expansion of bovine and porcine smooth muscle cells, bovine and porcine induced pluripotent, embryonic and mesenchymal stem cells.
Recombinant FGF2-G3 is used in B8 media (Kuo et al. 2020) and Beefy-9 media (Stout et al. 2023) for weekend free, high homogeneity induced pluripotent stem cell culture. FGF2-G3 also has applications in chemically defined stem cell and organoid culture media.
Bovine/porcine FGF2-G3 has a molecular weight of 16.3 kDa. This protein is carrier-free and tag-free to ensure purity with exceptional lot-to-lot consistency.
Download the Product Data Sheet for species reactivity, reconstitution instructions, bioactivity, purity, and additional product information.
Contact us for Quality and Safety documentation or for information on cell therapy grade growth factors and cytokines.
Product Features
- High purity thermostable bovine/porcine FGF2-G3
- 16.3 kDa (monomer)
- >98%, by SDS-PAGE quantitative densitometry
- Animal origin-free (AOF) and carrier protein-free
- Expressed in E. coli
- Manufactured in Qkine’s Cambridge, UK laboratories
- Lyophilized from Tris, NaCl, CyS, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured Applications:
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- Maintenance and expansion of bovine iPSC, ESC and primary cells
- Cellular agriculture and cultivated meat cell culture media optimization
RoosterBio is a distributor of Qkine growth factors and cytokines within the Unites States, for regional specific orders please contact us for more information.
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How do I store my proteins?
- Lyophilized store at -20°C or -80°C for up to 2 years
- Reconstituted store at -20 °C or -80 °C
- We recommend that single use aliquots are prepared to avoid freeze-thaw cycles
How do I reconstitute lyophilized protein?
- Check the resuspension solution recommendation on the product datasheet.*
- Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
- Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
- Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating. Do not vortex and avoid foaming.
- Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles, which can damage the proteins and reduce bioactivity. Store aliquots at -80°C (or -20°C).**
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors, so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml), adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).