Recombinant Bovine Vitronectin Protein
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Overview
Vitronectin is widely used in stem cell culture. It provides a defined environment than supports maintenance of pluripotency and is suitable for feeder-free culture, maintenance, differentiation, and reprogramming of stem cells.
Qkine bovine vitronectin is a high-purity animal origin-free recombinant protein with a molecular weight of 50.1 kDa. It is carrier-free and protein tag-free, ensuring exceptional lot-to-lot consistency. Qkine vitronectin is exceptionally high purity with industry-leading low residual endotoxins for highly reproducible culture of stem cells for cellular agriculture.
Download the Product Data Sheet for species reactivity, reconstitution instructions, bioactivity, purity, and additional product information.
Contact us for Quality and Safety documentation or for information on cell therapy grade growth factors and cytokines.
Product Features
- Highly pure recombinant bovine vitronectin protein (UniProt: Q3ZBS7)
- 50.1 kDa (monomer)
- >98%, by SDS-PAGE quantitative densitometry
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Qkine’s Cambridge, UK laboratories
- Lyophilized from PBS, mannitol and TCEP
- Resuspend in sterile-filtered water at 1 mg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured Applications:
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- Maintenance and expansion of bovine iPSC, ESC and primary cells
- Differentiation of human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal, neural lineages, and chondrocytes
- Promotion of cell adhesion and migration
- Stimulation of angiogenesis and vascular network development
- Organoid growth and proliferation
RoosterBio is a distributor of Qkine growth factors and cytokines within the Unites States, for regional specific orders please contact us for more information.
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How do I store my proteins?
- Lyophilized store at -20°C or -80°C for up to 2 years
- Reconstituted store at -20 °C or -80 °C
- We recommend that single use aliquots are prepared to avoid freeze-thaw cycles
How do I reconstitute lyophilized protein?
- Check the resuspension solution recommendation on the product datasheet.*
- Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
- Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
- Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating. Do not vortex and avoid foaming.
- Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles, which can damage the proteins and reduce bioactivity. Store aliquots at -80°C (or -20°C).**
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors, so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml), adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).