Recombinant Human Activin A PLUS Protein
Recommended Products
There are no recommended products for Recombinant Human Activin A PLUS Protein
Overview
Human recombinant activin A PLUS™ protein is an optimized biologically active truncation of the mature domain of human activin A protein. The EC50 and activity in stem cell culture of activin A PLUS™ is equivalent to the full mature domain activin A.
High purity 24 kDa dimer comprising truncated mature domain of activin A protein, animal origin-free (AOF) and carrier-protein free (CF). Recombinant activin A PLUS™ is designed to be manufactured at scale for cost-effective, large-scale stem cell culture applications.
Download the Product Data Sheet for species reactivity, reconstitution instructions, bioactivity, purity, and additional product information.
Contact us for Quality and Safety documentation or for information on cell therapy grade growth factors and cytokines.
Product Features
- High purity optimized mature domain of human activin A (Uniprot: P08476) – particularly suitable for bulk and scale-up stem cell culture applications
- 24 kDa (dimer)
- >98%, by SDS-PAGE quantitative densitometry
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Qkine’s Cambridge, UK laboratories
- Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured Applications
- Large scale stem cell expansion
RoosterBio is a distributor of Qkine growth factors and cytokines within the United States. For regional-specific orders, please contact us for more information.
Login to search Certificates of Analysis and Quality Control Briefs.
How do I store my proteins?
- Lyophilized store at -20°C or -80°C for up to 2 years
- Reconstituted store at -20 °C or -80 °C
- We recommend that single use aliquots are prepared to avoid freeze-thaw cycles
How do I reconstitute lyophilized protein?
- Check the resuspension solution recommendation on the product datasheet.*
- Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
- Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
- Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating. Do not vortex and avoid foaming.
- Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles, which can damage the proteins and reduce bioactivity. Store aliquots at -80°C (or -20°C).**
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors, so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml), adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).