Recombinant Human CNTF Protein
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Overview
Ciliary Neurotrophic Factor (CNTF) is a member of the IL-6 family of cytokines. CNTF plays a crucial role in developing and maintaining the nervous system, in particular the optic nervous system. It promotes the maintenance, differentiation, and survival of various neurons, glial cells, and retinal cells. CNTF has been used in vitro to initiate neural induction and differentiation. CNTF can be used to culture primary neurons and glial cells such as astrocytes and Schwann cells. It is also used to culture retinal cells and adipocytes.
Human CNTF has a molecular weight of 22.8 kDa. This protein is animal origin-free, carrier-free, tag-free, and non-glycosylated to ensure its purity with exceptional lot-to-lot consistency. Qk063 is suitable for the culture of reproducible and high-quality neurons and other relevant cells.
Download the Product Data Sheet for species reactivity, reconstitution instructions, bioactivity, purity, and additional product information.
Contact us for Quality and Safety documentation or for information on cell therapy grade growth factors and cytokines.
Product Features
- High purity species protein (Uniprot: P26441)
- 22.8 kDa (monomer)
- >98%, by SDS-PAGE quantitative densitometry
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Qkine’s Cambridge, UK laboratories
- Lyophilized from HEPES, NaCl
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured Applications:
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- Neural stem cell proliferation and neuronal differentiation
- Differentiation of iPSC derived neural progenitors to neurons
- Differentiation of iPSC-derived Schwann cells and astrocytes
- Culture of retinal cells
- Induction of axonal growth
- Neural stem cell research
RoosterBio is a distributor of Qkine growth factors and cytokines within the Unites States, for regional specific orders please contact us for more information.
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How do I store my proteins?
- Lyophilized store at -20°C or -80°C for up to 2 years
- Reconstituted store at -20 °C or -80 °C
- We recommend that single use aliquots are prepared to avoid freeze-thaw cycles
How do I reconstitute lyophilized protein?
- Check the resuspension solution recommendation on the product datasheet.*
- Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
- Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
- Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating. Do not vortex and avoid foaming.
- Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles, which can damage the proteins and reduce bioactivity. Store aliquots at -80°C (or -20°C).**
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors, so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml), adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).