Recombinant Human G-CSF Protein
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Overview
Granulocyte colony-stimulating factor (G-CSF) is a member of the hematopoietic growth factor family which plays a crucial role in the proliferation, differentiation, and maturation of committed progenitor cells to granulocytes, such as neutrophils.
Qkine G-CSF is a highly pure, bioactive protein produced in an animal origin-free expression system composed of 174 amino acids with a molecular weight of 18.6 kDa. Human and murine G-CSF are 73% identical at the amino acid level and show species cross-reactivity. Human G-CSF is carrier-free, tag-free, and non-glycosylated to ensure a pure and homogenous production of high-quality neutrophils and other relevant cell cultures with exceptional lot-to-lot consistency.
Download the Product Data Sheet for species reactivity, reconstitution instructions, bioactivity, purity, and additional product information.
Contact us for Quality and Safety documentation or for information on cell therapy grade growth factors and cytokines.
Product Features
- High purity human G-CSF protein (Uniprot: P09919-2)
- 18.6 kDa (monomer)
- >98%, by SDS-PAGE quantitative densitometry
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Qkine’s Cambridge, UK laboratories
- Lyophilized from Tris, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured Applications:
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- Generation of iPSC-derived neutrophils
- Induction of cardiomyocytes from iPSCs
- Mobilization of bone marrow cells for wound healing applications
- Regulation of ZO-1 expression in brain microvascular endothelial cells
- Maintenance of isolated neutrophils
- Proliferation of neutrophils
RoosterBio is a distributor of Qkine growth factors and cytokines within the Unites States, for regional specific orders please contact us for more information.
Login to search Certificates of Analysis and Quality Control Briefs.
How do I store my proteins?
- Lyophilized store at -20°C or -80°C for up to 2 years
- Reconstituted store at -20 °C or -80 °C
- We recommend that single use aliquots are prepared to avoid freeze-thaw cycles
How do I reconstitute lyophilized protein?
- Check the resuspension solution recommendation on the product datasheet.*
- Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
- Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
- Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating. Do not vortex and avoid foaming.
- Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles, which can damage the proteins and reduce bioactivity. Store aliquots at -80°C (or -20°C).**
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors, so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml), adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).