Recombinant Human GDF-5 Protein
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Overview
Growth differentiation factor 5 (GDF-5) plays a crucial role during embryonic development and tissue homeostasis and is specifically involved in the development of the skeletal system. Recombinant human GDF-5 protein is commonly used for the differentiation and maintenance of induced pluripotent stem cells, embryonic stem cells, or bone marrow-derived mesenchymal stem cells into osteoblasts and chondrocytes.
Human GDF-5 is a dimer with a molecular weight of 27 kDa. This protein is animal origin-free, carrier protein-free, and tag- free with exceptional lot-to-lot consistency. GDF-5 is suitable for reproducible and high-quality chondrocytes and osteoblasts.
Download the Product Data Sheet for species reactivity, reconstitution instructions, bioactivity, purity, and additional product information.
Contact us for Quality and Safety documentation or for information on cell therapy grade growth factors and cytokines.
Product Features
- High-purity human GDF-5 (UniProt number: P43026)
- 27 kDa (monomer)
- >98%, by SDS-PAGE quantitative densitometry
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Qkine’s Cambridge, UK laboratories
- Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured Applications:
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- Directed chondrogenic differentiation of mesenchymal stem cells
- Generation of iPSC-derived osteoblasts and chondrocytes
- Mesenchymal stem cell research
- Differentiation of adipose-derived stromal cells to osteogenic lineages
RoosterBio is a distributor of Qkine growth factors and cytokines within the Unites States, for regional specific orders please contact us for more information.
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How do I store my proteins?
- Lyophilized store at -20°C or -80°C for up to 2 years
- Reconstituted store at -20 °C or -80 °C
- We recommend that single use aliquots are prepared to avoid freeze-thaw cycles
How do I reconstitute lyophilized protein?
- Check the resuspension solution recommendation on the product datasheet.*
- Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
- Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
- Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating. Do not vortex and avoid foaming.
- Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles, which can damage the proteins and reduce bioactivity. Store aliquots at -80°C (or -20°C).**
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors, so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml), adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).