Recombinant Human IL-34 PLUS™ Protein
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Overview
Interleukin-34 (IL-34) is a cytokine involved in regulating the differentiation, survival, and activation of monocytes, macrophages, and osteoclasts. IL-34 plays a key role for the development and maintenance of tissue-resident macrophages such as Langerhans cells in the skin and microglia in the brain. Recombinant IL-34 can be used in the differentiation of induced pluripotent stem cells (iPSC) to microglia.
Qkine have optimized the sequence and manufacture of IL-34 PLUS™ to produce a highly and consistently bioactive protein (patent pending GB 2514413.0). Qk091 has a molecular weight of 25 kDa as a monomer and is supplied as a non-covalent dimer, highly pure, animal origin-free, tag free and carrier free for reproducible microglial differentiation and immune cell culture.
Download the Product Data Sheet for species reactivity, reconstitution instructions, bioactivity, purity, and additional product information.
Contact us for Quality and Safety documentation or for information on cell therapy grade growth factors and cytokines.
Product Features
- High purity optimized human IL-34 protein (Uniprot: Q6ZMJ4)
- 25 kDa (monomer)
- >98%, by SDS-PAGE quantitative densitometry
- Animal origin-free (AOF) and carrier protein-free
- Expressed in E. coli
- Manufactured in Qkine’s Cambridge, UK laboratories
- Lyophilized from acetonitrile, TFA
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured Applications:
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- Polarization of anti-inflammatory or M2 macrophage
- Generation of iPSC-derived microglia and Langerhans cells
- Osteoclast culture to study osteoporosis and bone disorders
RoosterBio is a distributor of Qkine growth factors and cytokines within the Unites States, for regional specific orders please contact us for more information.
Login to search Certificates of Analysis and Quality Control Briefs.
How do I store my proteins?
- Lyophilized store at -20°C or -80°C for up to 2 years
- Reconstituted store at -20 °C or -80 °C
- We recommend that single use aliquots are prepared to avoid freeze-thaw cycles
How do I reconstitute lyophilized protein?
- Check the resuspension solution recommendation on the product datasheet.*
- Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
- Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
- Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating. Do not vortex and avoid foaming.
- Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles, which can damage the proteins and reduce bioactivity. Store aliquots at -80°C (or -20°C).**
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors, so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml), adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).