Recombinant Human Nodal Protein
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Overview
Nodal is a transforming growth factor β (TGF-β) family member which has an essential role in embryonic development. Nodal signaling controls endoderm and mesoderm germ layer specification whilst preventing neuroectoderm formation. It has also been implicated in cancer metastasis. Recombinant nodal has been found to maintain pluripotency in embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs).
Qkine recombinant human nodal has been manufactured to be completely animal origin-free with reliable and consistent bioactivity for the reproducible maintenance of pluripotency in stem cells. Recombinant nodal is a 25.9 kDa homodimer, tag and carrier free.
Download the Product Data Sheet for species reactivity, reconstitution instructions, bioactivity, purity, and additional product information.
Contact us for Quality and Safety documentation or for information on cell therapy grade growth factors and cytokines.
Product Features
- High purity human nodal protein (UniProt: Q96S42)
- 25.9 kDa (homodimer)
- >98%, by SDS-PAGE quantitative densitometry
- Animal origin-free (AOF) and carrier protein-free
- Expressed in E. coli
- Manufactured in Qkine’s ISO 9001:2015 facility in Cambridge, UK
- Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured Applications
- iPSC-derived mesoderm differentiation
- Differentiation of iPSC into endoderm
- Cancer research
- Maintenance of pluripotency in iPSC
- Maintenance of undifferentiated embryonic stem cells
RoosterBio is a distributor of Qkine growth factors and cytokines within the United States. For regional-specific orders, please contact us for more information.
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How do I store my proteins?
- Lyophilized store at -20°C or -80°C for up to 2 years
- Reconstituted store at -20 °C or -80 °C
- We recommend that single use aliquots are prepared to avoid freeze-thaw cycles
How do I reconstitute lyophilized protein?
- Check the resuspension solution recommendation on the product datasheet.*
- Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
- Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
- Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating. Do not vortex and avoid foaming.
- Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles, which can damage the proteins and reduce bioactivity. Store aliquots at -80°C (or -20°C).**
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors, so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml), adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).