Recombinant Mouse FGF-8b Protein
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Overview
Fibroblast growth factor 8b (FGF-8b) is a member of the FGF family involved in the regulation of embryogenesis, cellular proliferation, differentiation, and migration. FGF-8b is commonly used for the differentiation of induced pluripotent stem cells into neural cell types and brain organoid cultures. FGF-8b is a spliced form of FGF-8, a heparin-binding protein that targets mammary and non-mammary cells expressing the FGF receptors.
Qkine mouse FGF-8b is a 22.5 kDa highly pure, bioactive recombinant protein produced in an animal origin-free expression system. This protein is carrier-free, tag-free and non-glycosylated to ensure a pure, homogenous protein with exceptional lot-to-lot consistency, suitable for enhanced reproducibility and physiologically relevant cultures.
Download the Product Data Sheet for species reactivity, reconstitution instructions, bioactivity, purity, and additional product information.
Contact us for Quality and Safety documentation or for information on cell therapy grade growth factors and cytokines.
Product Features
- High purity FGF-8b protein (Uniprot: Q3U1V5)
- 22.5 kDa
- >98%, by SDS-PAGE quantitative densitometry
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Qkine’s Cambridge, UK laboratories
- Lyophilized from HEPES, NaCl, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured Applications
- Differentiation of midbrain dopaminergic neurons
- Neurite outgrowth from spiral ganglion neurons
- Generation of iPSC-derived midbrain organoids
RoosterBio is a distributor of Qkine growth factors and cytokines within the United States. For regional-specific orders, please contact us for more information.
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How do I store my proteins?
- Lyophilized store at -20°C or -80°C for up to 2 years
- Reconstituted store at -20 °C or -80 °C
- We recommend that single use aliquots are prepared to avoid freeze-thaw cycles
How do I reconstitute lyophilized protein?
- Check the resuspension solution recommendation on the product datasheet.*
- Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
- Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
- Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating. Do not vortex and avoid foaming.
- Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles, which can damage the proteins and reduce bioactivity. Store aliquots at -80°C (or -20°C).**
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors, so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml), adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).