Recombinant Rat BDNF Protein
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Overview
Brain-derived neurotrophic factor (BDNF) is a member of neurotrophin family and plays a crucial role in neural development, maintenance, and function. It stimulates neurogenesis and is also a major regulator of synaptic plasticity and neuroprotection. It is used to maintain neurons and differentiate mature pluripotent stem cell-derived neural progenitors to cortical and motor neurons and cortical organoids.
Recombinant rat BDNF protein has a molecular weight of 14 kDa. This protein is animal origin-free, carrier-free, tag-free, and non-glycosylated to ensure its purity with exceptional lot-to-lot consistency. Qkine BDNF is suitable for the culture of reproducible and high-quality cortical and motor neurons and cortical organoids.
Download the Product Data Sheet for species reactivity, reconstitution instructions, bioactivity, purity, and additional product information.
Contact us for Quality and Safety documentation or for information on cell therapy grade growth factors and cytokines.
Product Features
- High purity BDNF protein (Uniprot: P23363)
- 14 kDa
- >98%, by SDS-PAGE quantitative densitometry
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Qkine’s Cambridge, UK laboratories
- Lyophilized from acetonitrile, TFA
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured Applications
- Differentiation of midbrain dopaminergic neurons
- Differentiation of iPSC derived neural progenitors to neurons
- Generation of iPSC-derived midbrain organoids
RoosterBio is a distributor of Qkine growth factors and cytokines within the United States. For regional-specific orders, please contact us for more information.
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How do I store my proteins?
- Lyophilized store at -20°C or -80°C for up to 2 years
- Reconstituted store at -20 °C or -80 °C
- We recommend that single use aliquots are prepared to avoid freeze-thaw cycles
How do I reconstitute lyophilized protein?
- Check the resuspension solution recommendation on the product datasheet.*
- Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
- Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
- Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating. Do not vortex and avoid foaming.
- Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles, which can damage the proteins and reduce bioactivity. Store aliquots at -80°C (or -20°C).**
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors, so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml), adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).