Recombinant Salmon FGF-2 (145 aa) Protein
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Overview
Recombinant salmon FGF-2 protein 145 aa (bFGF/basic FGF) for the development of optimized serum-free culture media for species-specific Atlantic salmon (Salmo salar) in cellular agriculture protocols and veterinary research applications. This shorter form of FGF-2 is used in comparative cell culture media optimization studies alongside Qk103, the 154 aa form of salmon FGF-2. FGF-2 is used extensively in the maintenance and proliferation of induced pluripotent (iPSC) and embryonic stem cells (ESC) and for enhancement of proliferation in primary salmon cell culture. Receptor binding affinity and efficacy may differ depending on each species. Using a species-specific growth factor enhances receptor binding affinity, resulting in a lower concentration required in culture.
High purity 16.1 kDa salmon FGF-2 / bFGF protein, animal origin-free (AOF) and carrier-protein free (CF).
Download the Product Data Sheet for species reactivity, reconstitution instructions, bioactivity, purity, and additional product information.
Contact us for Quality and Safety documentation or for information on cell therapy grade growth factors and cytokines.
Product Features
- High purity salmon protein (Uniprot number: XP_014067501.1)
- 16.1 kDa (monomer)
- >98%, by SDS-PAGE quantitative densitometry
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Qkine’s Cambridge, UK laboratories
- Lyophilized from Tris, NaCl, CyS, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured Applications:
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- Expansion of salmon pluripotent, embryonic and mesenchymal stem cells
- Cellular agriculture and cultivated meat cell culture media optimization
- Serum-free media development
- Cellular proliferation, migration and survival
RoosterBio is a distributor of Qkine growth factors and cytokines within the Unites States, for regional specific orders please contact us for more information.
Login to search Certificates of Analysis and Quality Control Briefs.
How do I store my proteins?
- Lyophilized store at -20°C or -80°C for up to 2 years
- Reconstituted store at -20 °C or -80 °C
- We recommend that single use aliquots are prepared to avoid freeze-thaw cycles
How do I reconstitute lyophilized protein?
- Check the resuspension solution recommendation on the product datasheet.*
- Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
- Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
- Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating. Do not vortex and avoid foaming.
- Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles, which can damage the proteins and reduce bioactivity. Store aliquots at -80°C (or -20°C).**
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors, so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml), adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).