Engineered from the ground up, RoosterGEM is a complete media built to solve key challenges in both viral and non-viral MSC and primary cell engineering.
Traditionally, genetic modification of primary cells has low efficiency of transfer requiring increased viral and non-viral agent costs, with significant time and resources allocated to media and process optimization.
RoosterGEM isformulated to increase genetic engineering efficiencies, enabling a reduction in key raw material costs and more readily meet regulatory guidelines. RoosterGEM 50X is an in-process transduction enhancer for use in workflows, such as T-Cells, requiring native media with activators.
When paired with RoosterBio’s industrialized supply chain of high-volume Xeno-free hMSCs, ultra-productive expansion media, and cGMP-compatible processes – you enlist an end-to-end Genetic Engineering MSC-platform to streamline your transient or integrated gene transfer, de-risk your product & process development, and streamline your path to clinical translation.
Product Features
200 mL RoosterGEM™ [part no. M40200] complete medium.
1 ml RoosterGEM 50X format [part no. M40001] for use as an in-process additive to native media.
Increased Viral and Non-viral primary cell engineering efficiency.
Supported by simplified protocols to streamline process optimization.
Contact RoosterBio for more information on scalable 2D or 3D bioreactor genetically modified cell bank process development
*To maximize final product yield contact RoosterBio for information on low PDL cell banks.
Lentiviral transduction efficiency at Low MOI (rLV.EF1.ZsGreen1-9, MOI=2) was increased by 3 to 5-fold compared to traditional serum and xeno-free media systems. This reduction in MOI dramatically reduced lentiviral associated costs.
Increased transduction efficiencies were achieved in RoosterBio's Xeno-free RoosterVial hMSCs from multiple donors and tissue sources.
Increased transient transfection efficiencies were achieved in hMSCs using RoosterGEM.
RoosterGEM-50X as an in-process reagent for T-Cell engineering in native media.
