An MSC-specific, xeno-free (XF) bioreactor feed

  • SKU/Catalog No: SU-023
  • Unit Size: 10 mL
  • Price: $298.00
Engineered to clear out costly media exchanges, taking media productivity to a whole new level – and making you an MSC bioreactor expert, overnight.


Contact your RoosterBio representative for information on cGMP formats of RoosterReplenish-MSC.

RoosterReplenishTM-MSC-XF is a concentrated bioreactor feed for use in a fed-batch bioreactor process, enhancing media productivity to drive a radically cost-effective process that is less labor-intensive in small- and large-scale expansions of hMSCs in 3D bioreactors. RoosterReplenishTM-MSC-XF is designed to achieve high cell growth in microcarrier-based suspension cultures when paired with our xeno-free (XF) hMSCs (derived from multiple tissue types) and paired bioprocess media (RoosterNourish™-MSC-XF). Using RoosterNourish™-MSC-XF and RoosterReplenishTM-MSC-XF in a fed-batch system not only increases culture yield, it also minimizes the time required for media preparation, eliminates media exchange, and reduces the contamination risk associated with in-process manipulation. RoosterReplenishTM-MSC-XF boosts media productivity (millions of cells produced per Liter of media consumed) by up to 70%. Each 10 mL bottle of RoosterReplenishTM-MSC-XF can be used to supplement 500 mL of RoosterNourishTM-MSC-XF, with analogous version available for cGMP manufacturing.

Product Features

  • Enables a fed-batch suspension hMSC culture process. This feed is added on Day 3 of bioreactor culture to provide additional mitogenic factors to the culture.
  • Streamlines culture processes & reduces processing time and labor.
  • Minimizes media usage, reducing total cost associated with hMSC culture scale-up, and boosts media productivity by up to 70%.
  • Maintains stable nutrient and waste bio-profiles through 7 days of culture; comparable to batch culture.
  • Incorporates translation-friendly processes and components into your workflow.
  • Eliminates the media efficiency optimization step in small- and large-scale stem/stromal cell biomanufacturing process development – shortening development timelines, increasing cell lot size, and streamlining biomanufacturing steps for those on the path to commercial scale production.
  • Mitigates risk of contamination by minimizing in-process culture manipulation.

Intended Use: Research Use Only

Fig 1: A comparison of XF hMSC cell densities in bioreactor culture using batch and fed-batch processes. RoosterVial™-hBM-XF (Xeno-free human bone marrow-derived mesenchymal stem cells – RoosterVial™-hBM-10M-XF – were cultured in suspension on microcarriers in RoosterNourish™-MSC-XF and fed with RoosterReplenish™-MSC-XF on Day 3, and compared to batch culture.
Fig 2: Fed-batch culture using RoosterReplenish™-MSC-XF is the most efficient and productive culture feeding regimen, outperforming either 1/2 media exchange or batch culture in media productivity by over 70%, and achieving up to 350 million (M) cells/L. This productivity is >17 times the media productivity of traditional hMSC flask-based cultures of ~20M cells/L, and is the central driving force for cost reduction in bioreactor-based manufacturing. Note: Total cell yield is donor-dependent.
Product Documentation

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