RoosterVial-hTERT-hCP-XF (C51001CP) and RoosterVial-hTERT-hBM-XF (C52001BM) are xeno-free human Mesenchymal Stem/Stromal Cells isolated from either placental chorionic plate (hCP-MSCs) or bone marrow tissue (hBM-MSCs) and life span-extended via ectopic hTERT expression.
Each lot is held to strict release criteria covering performance, identity, and safety. Working cell banks were continuously cultured in RoosterNourish-MSC-XF (K82016) with Geneticin (G418) antibiotic selection for more than 25 population doublings without exhibiting reduced growth or replicative senescence. Efficiently manufacture trillions of cells by leveraging a media exchange free process that reduces doubling time while maintaining MSC identity and functional characteristics.
Product Features
1M cryopreserved, XF hTERT-modified hMSCs
hTERT modified via non-viral plasmid gene transfer
10-fold expansion per 4 to 5 day passage in RoosterNourish™-MSC-XF
>25 population doublings from working cell bank
Expansion protocols supplied
Comprehensive hMSC identity and functional characterization data package
Intended Use: Research Use Only
License Requirements:
License Required for Pharmaceutical Research: RoosterBio out-licenses telomerized cells for use in for-profit pharmaceutical research, where a flat $5,500 fee allows customers to evaluate our hTERT-modified cell in their laboratories for a period of 6 months, after which an annual license fee is due. For-profit pharmaceutical customers must agree to the conditions in our license agreement prior to shipping. Please email info@roosterbio.com for license agreement.
RoosterVial-hTERT-CP working cell banks grown in XF RoosterNourish expansion media (left) for more than 10 passages and 25 populations doublings. Working cell banks (right) exhibit hall mark MSC protein surface marker expression.
RoosterVial-hTERT-BM working cell banks grown in XF RoosterNourish expansion media (left) for more than 10 passages and 25 populations doublings. Working cell banks (right) exhibit hall mark MSC protein surface marker expression.
RoosterVial-hTERT-CP working cell banks exhibit functional (left) MSC angiogenic cytokine secretion profile and (right) immunomodulatory activity.
RoosterVial-hTERT-BM working cell banks exhibit functional (left) MSC angiogenic cytokine secretion profile and (right) immunomodulatory activity.
RoosterVial-hTERT pre-expanded for 5 days in 3D bioreactors with XF RoosterNourish expansion media and RoosterReplenish™ bioreactor feed, then switched to RoosterCollect™-EV for 5 days of exosome collection.
