Neonatal Dermal Fibroblasts

Overview

RoosterVial™-hDF-1M-XF are off-the-shelf neonatal foreskin-derived dermal fibroblasts that provide a consistent, well-characterized research and development tool.

Each vial consists of 1M cells manufactured to a consistent population doubling level. Lot-specific expansion performance testing supports >10-fold expansion within 4–5 days (in RoosterNourish™-MSC-XF) and >20 additional population doublings from thaw.

RoosterVial™-hDF-1M-XF are compatible with established scalable 2D and 3D protocols to easily manufacture billions of cells or trillions of hDF-EVs.

RoosterVial™-hDF-XF is also available as part of RoosterKit™-hDF-1M-XF (K83155DF) which pairs 1 vial of cells with RoosterNourish™-MSC-XF, RoosterVial™-hDF-DEV+ for preclinical development, and RoosterVial™-hDF-CC for clinical manufacturing.

Product Information Sheet

Product Features

• 1M cryopreserved xeno-free cells per vial
• Manufactured from normal human neonatal foreskin donor tissue
• Release testing includes: cell count, viability, and flow cytometry for fibroblast surface marker expression (S100A4, CD90, CD142) and negative controls (keratin, CD31).
• Safety testing includes: sterility, endotoxin, and mycoplasma
• Characterization testing includes: population doubling level (PDL)
• >10-fold expansion in 4 to 5 days when cultured with RoosterNourish^™-MSC-XF
• Easy-to-follow expansion protocols for hDF and hDF-EV production in 2D and 3D:
• 2D Expansion Protocol 
• 125ml Spinner Flask hDF Protocol*
• 125ml Spinner Flask hDF-EV Protocol*
• Expansion protocols do not require media exchanges or surface coatings

Intended Use: Research Use Only