RoosterVial™-hDF-1M-XF are Primary Human Dermal Fibroblasts (hDF) isolated from normal neonatal foreskin and expanded to 1M cells per vial at a PDL of 15-20 to serve as working cell banks for R&D and product development needs.
Each lot is performance qualified to expand >10-fold within 4 to 5 days (in RoosterNourish™-MSC-XF ) and supports >20 additional population doublings from thaw. Accompanying QC information includes identity characterization for hallmark fibroblast surface markers (S100A4, CD90, CD142) along with negative controls (keratin, CD31).
When paired with RoosterNourish™-MSC-XF’s media-exchange-free expansion system, RoosterVial™-hDF-XF cells can efficiently scale from 2D to 3D utilizing easy-to-follow protocols and can reproducibly produce millions to billions of fibroblasts. Together, these optimized platforms save months of development time and costs while outperforming competing systems.
RoosterVial™-hDF-XF is available in 1M cell vials or as part of RoosterKit™-hDF-1M-XF (K83155DF) which pairs 1 vial of cells with RoosterNourish™-MSC-XF.
Product Features
1M cryopreserved XF hDFs from normal neonatal foreskin
Supported by first-in-class hDF identity and expansion characterization
> 10-fold, media exchange free expansion in 4 to 5 days when cultured with RoosterNourish™-MSC-XF
Supports >20 additional population doublings
Easy-to-follow expansion protocols for hDF and hDF-EV production in 2D and 3D:
Contact us for GMP or GMP-derived fibroblast cell banks.
Intended Use: Research Use Only
XF RoosterVial-hDF (C55001DF) cultured in RoosterNourish-MSC-XF expansion profile (left) and hDF specific cell surface marker expression (right).
Cumulative population doublings of XF RoosterVial-hDF (C55001DF) (left) and doubling time (right) over 7 passages from working cell bank, when cultured in a media exchange free process with RoosterNourish-MSC-XF.
Utilizing a 3D bioreactor system, XF RoosterVial-hDF (C55001DF) reaches 1E6 cells/ml in 6 Days (left) and >1E10 particles/ml (right) when cultured in RoosterNourish-MSC-XF (K82016) paired with RoosterReplenish™ (SU-023) and RoosterCollect™ (M2001).
