RoosterKit™-hBM-1M contains 500 mL of RoosterNourish™-MSC (KT-001) and 1 vial of 1M cryopreserved hBM-MSC (RoosterVial™-hBM-1M, Part No. MSC-003). RoosterNourish™- MSC is an affordable, highly-productive traditional bioprocess medium* for the rapid expansion of human mesenchymal stem/stromal cells (hMSCs) from multiple tissue sources. This complete kit, with included RoosterBio® protocols, supports in vitro expansion of hMSCs in a 2D batch process to reproducibly generate 10X hMSCs expansion in less than 1 week. All RoosterBio hBM-MSCs meet the ISCT minimal criteria for defining MSCs and are released based on hMSC identity (cell surface marker expression), expansion, viability, sterility, and differentiation potential. Additional QC Brief contains industry-leading functional characterization, including immunomodulatory potential (IDO assay) and angiogenic cytokine secretion profile.
This kit has been used to generate critical cellular raw materials to accelerate top research and publications in over 25 countries. Designed to quickly test hBM-MSCs in your research, RoosterKit™-hBM-1M can efficiently generate 100M hMSCs to “Radically Simplify” your research.
* RoosterNourish™-MSC contains recombinant growth factors, high-quality small molecules and low levels of quality bovine serum
Product Features
1M cells cryopreserved human Bone Marrow-Derived MSCs
1 RoosterNourishTM-MSC media kit (KT-001)
Complete RoosterBio expansion protocol and process recommendations
> 10 fold expansion in less than 1 week
Built-in batch culture manufacturing process capable of generating >100 M cells in 8 to 10 days
Multiple donors available for screening
1st in class CofA and QC Brief complete with lot-specific functional hMSC characterization
RoosterVial™-hBM-1M (MSC-003) cultured in RoosterNourish™-MSC (KT-001) have a fibroblastic cell morphology (left) and express hMSC cell surface markers (right).
Immunomodulatory activity (left, IDO activity increase in response to IFNy) and angiogenic cytokine secretion of a representative RoosterVial™-hBM-1M (MSC-003) lot (right).
Trilineage differentiation of RoosterVial™-hBM-1M (MSC-003) of a representative MSC-003 lot. Adipogenesis, left; Osteogenesis, middle; Chondrogenesis, right.
RoosterBio cell and media products outperform the leading industry products and significantly decrease time and cost associated with hMSC manufacturing
I have tried different companies cells, the major problem for me was the cell growth really slow. This starting kit works so well. It only take two or three days to go the next passage, and there is no affect to the cell morphology. This should be the best cell I got. It's really worth to try, and will save you a lot of time.
These cells look much more healthy and definitely grow much faster than other MSCs I've tried. Also the media does seem to work well since it significantly helps proliferation when culturing other MSCs as well! Definitely worth trying these cells & the media!
The expansion of the cells using their High Performance Media Kit was extremely simple, effective and provided a fantastic number of cells relatively quickly. While these cells performed great in DMEM and aMEM, compared to others, the RoosterBio media was second to none especially when thawing out aliquots - next to no cell death. About to purchase again without question!
As a student with no prior experience in cell culture or expansion, I was easily able to turn a frozen vial of 0.75Million Rooster Bio MSC cells into 15Million cells in just 4 days. The protocol they gave for expanding and culturing the cells were very easy to follow along with. After expansion, the cells were extremely viable and they looked nice and healthy!
The cells stay robust for a long time without affecting the morphology. They proliferate at a rapid rate than those I have used till now. The proprietary media helps in expanding the cells exponentially. These cells are best for experiments requiring high number of cells. Worth a try and then I am sure you wont regret!
I have more than 10 yrs experience growing MSCs and I have never seen MSCs look this good and grow this well. Whatever magic potion RoosterBio has developed really works. The cells are growing well and giving us great results in all of the tests we have tried so far. Highly recommend these cells!
The starter kit works just as described. Wonderful experience, especially the expertise of the researchers at RoosterBio who provide instant and professional technical support. Thank you!
