RoosterCollect-EV Pro™ is a translation-friendly, low particulate, chemically defined Extracellular Vesicle (EV) collection media kit designed to increase total hMSC-EV yields. For use post-cell expansion, this medium supports cell health during hMSC-based EV collection to elongate the collection window, increase EV yields, and reduce variability in 3D Bioreactor as well as 2D flask culture systems.
As part of RoosterBio’s EV Production System – a bioprocess engine for rapid EV production – each RoosterCollect EV Pro kit is engineered to support RoosterBio’s scalable and translation-friendly Development Grade xeno-free (XF) product portfolio, including high volume hMSCs and bioprocess media. Across tissue types, this low-particulate medium and paired 2D batch or 3D bioreactor fed-batch bioprocess scales with your stage of product development – ensuring unparalleled hMSC-EV production.
Industrialize your hMSC-EV supply chain with a complete extracellular vesicle (EV) production workflow. Seamlessly transition from rapid cell expansion to dependable high-yield EV collection.
Product Features
500 mL RoosterCollect™-EV [part no. M2001] and 10 mL EV Pro™ [part no. S44002] supplement.
Low-particulate. Xeno-free. Chemically defined.
For streamlined, scalable hMSC-based EV production in 3D Bioreactor and 2D flask culture.
Supports increased efficiencies and EV productivity.
Ensures end product purity.
Supported by EV characterization as recommended by ISEV.
See included process recommendations or contact RoosterBio for protocols to support 2D and 3D bioreactor cell expansion and EV collection.
Intended Use: Research Use Only
RoosterCollect EV Pro™ is a low particle, low noise medium engineered for increased EV yields.
RoosterCollect EV Pro™ increased EV yields in both 3D bioreactor and 2D flask culture when used alongside RoosterBio's bioprocess media, high-volume hMSCs, and recommended (2D and 3D) hMSC expansion and hMSC-EV collection protocols.
How long can I store RoosterCollect EV Pro for, once prepared?
RoosterCollect EV Pro should be stored at 4°C and used within two weeks after it is prepared.
How do I warm RoosterCollect EV Pro?
It is recommended that RoosterCollect EV Pro is warmed at room temperature protected from light. Studies show warming at 37°C for extended periods of time can negatively impact cell growth and EV production in RoosterCollect EV Pro.
How long should I use RoosterCollect EV Pro for?
RoosterCollect EV Pro increases the amount of time that cells can be cultured in an EV Collection media by supporting cell health. We have cultured our cells in it for up to 6 additional days after the cells are initially grown in RoosterNourish-MSC-XF (part no. KT-016) and observe increased particle number each day. However, cell growth does start to plateau around 4 additional days of culture, so it is up to the customer to fully optimize the process and determine the culture time that works best for their application.
Will RoosterCollect EV Pro work with my cell type?
RoosterCollect EV Pro and the recommended protocol were developed with RoosterBio MSCs, but it may work well with other cell types. We recommend testing it out in your system.
