3D Bioreactor Media Kit

An MSC-specific, xeno-free (XF) media platform for fed-batch bioreactor expansion.

  • SKU/Catalog No: K86111
  • Components: M22520; SU-016; SU-023
  • Unit Size: 500mL bottle of RoosterBasal™2.0-CC, 10mL bottle of RoosterBooster™-MSC-XF, and 10mL bottle of RoosterReplenish™-MSC-XF
  • Price: $745.00
A media kit engineered to remove media exchanges from your 3D bioreactor MSC and EV manufacturing.


RoosterBio’s 3D Bioreactor Media Kit is an all-in-one system for 3D bioreactor expansion of MSCs and iPSC-derived MSCs. Engineered for fed-batch expansion to remove costly media exchanges, enhance overall productivity, and reduce in-process contamination risks;  this product has been vetted in a variety of commercially available bioreactor technologies to simplify integration into your program.

Comprised of RoosterNourishTM-MSC-XF expansion media and RoosterReplenishTM-MSC-XF concentrated bioreactor feed, this system reduces media costs by up to 70%.  Pair with one of our EV Collection Products to seamlessly transition from MSC production to exosome collection.

GMP CliniControl products are available for your clinical manufacturing needs. Contact your RoosterBio representative for information on cGMP Bioreactor Media options for large scale (3L to >50L) MSC or EV Production.

Product Features

  • Xeno-Free
  • Enables 3D hMSC manufacturing free of media exchange.
  • Each 10 mL bottle of RoosterReplenishTM-MSC-XF supplements  500 mL of RoosterNourishTM-MSC-XF to maintain stable nutrient and waste bio-profiles throughout culture.
  • Detailed protocols for easy transition to 3D MSC/EV production:
    • Spinner Flasks MSC | EV
    • PBS Mini MSC
    • AMBR250 MSC | EV
    • Contact us for additional larger scale protocols in a variety of platforms (from 3L to >50L)
  • Minimizes media usage, reducing total cost associated with hMSC culture scale-up by up to 70%
  • Mitigates risk of contamination by minimizing in-process culture manipulation.

Intended Use: Research Use Only

Fig 1: A comparison of XF hMSC cell densities in bioreactor culture using batch and fed-batch processes. RoosterVial™-hBM-XF (Xeno-free human bone marrow-derived mesenchymal stem cells – RoosterVial™-hBM-10M-XF – were cultured in suspension on microcarriers in RoosterNourish™-MSC-XF and fed with RoosterReplenish™-MSC-XF on Day 3, and compared to batch culture.
Fig 2: Fed-batch culture using RoosterReplenish™-MSC-XF is the most efficient and productive culture feeding regimen, outperforming either 1/2 media exchange or batch culture in media productivity by over 70%, and achieving up to 350 million (M) cells/L. This productivity is >17 times the media productivity of traditional hMSC flask-based cultures of ~20M cells/L, and is the central driving force for cost reduction in bioreactor-based manufacturing. Note: Total cell yield is donor-dependent.
Product Documentation Scientific Resources Blog Articles